[28] Enzymatic deglycosylation of glycoproteins

摘要: Publisher Summary Deglycosylation of glycoproteins is important in elucidation structure, function, and biosynthesis biologically significant glycoproteins. Enzymatic deglycosylation can be brought about by exoglycosidases either used sequentially or a mixture endoglycosidases. The chapter discusses the conditions processes Denatured are more susceptible to hydrolysis with these enzymes than their native conformation. Complete conformation can, however, achieved using much higher concentrations enzyme. precise for including enzyme concentration incubation time vary substrate and, therefore, should determined each unknown glycoprotein separately. Despite discovery several endoglycosidases that have facilitated investigations on structure carbohydrates, there still remains paucity broad specificity cleave carbohydrates from forms. There need continue efforts search new endoenzymes.