Acanthoic acid from Acanthopanax koreanum protects against liver injury induced by tert-butyl hydroperoxide or carbon tetrachloride in vitro and in vivo.

摘要: The aim of this study was to investigate the protective effect acanthoic acid, a diterpene isolated from root bark Acanthopanax koreanum, on liver injury induced by either tert-butyl hydroperoxide (tBH) or carbon tetrachloride in vitro and vivo. In vitro, cellular leakage lactate dehydrogenase (LDH) following treatment with 1.5 mM tBH for 1 h, significantly inhibited co-treatment acid (25 5 microg/mL) ED (50) 2.58 microg/mL (8.5 microM). LDH one hour 2.5 CCl (4) 4.25 (14.1 Co-treatment generation intracellular reactive oxygen species (ROS) glutathione (GSH) depletion (4). Acanthoic pretreatment (100 mg/kg per day four consecutive days, p. o.) reduced levels aspartate transaminase alanine acute models tetrachloride. Treatment mg/kg, at 6, 24, 48 hours after subcutaneous injection serum. Histological observations revealed that fatty changes, hepatocyte necrosis inflammatory cell infiltration (4)-injured were improved upon acid. vivo not able modify CYP2E1 activity protein expression microsomes dose used, showing hepatoprotective mediated through inhibition bioactivation. From results above, had against tBH- (4)-induced hepatotoxicity