Acanthoic acid, a diterpene in Acanthopanax koreanum, protects acetaminophen-induced hepatic toxicity in mice

摘要: Abstract The protective effect of a diterpenoid acanthoic acid (AA) isolated from Acanthopanax koreanum Nakai was investigated in acetaminophen (APAP)-induced hepatic toxicity. Drug-induced hepatotoxicity induced by an intraperitoneal (i.p.) injection 300 mg/kg (sub-lethal dose) APAP. Pretreatment with AA (50 and 100 mg/kg) orally 2 h before the APAP administration attenuated APAP-induced acute increase serum aspartate aminotransferase (AST), alanine (ALT) activites, replenished depleted glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), peroxidase (GSH-Px) activities, decreased malondialdehyde (MDA) level considerably reduced histopathological alterations manner similar to silymarin (Sily). Immunohistochemical analyses also demonstrated that could reduce appearance necrosis regions as well caspase-3 hypoxia inducible factor-1α (HIF-1α) expression liver tissue. Our results indicated protected tissue oxidative stress elicites damage suggestes protection mechanism would relate antioxidation factor on hepatotoxicity.