Circulating tumor cell isolation: the assets of filtration methods with polycarbonate track-etched filters.

摘要: Circulating tumor cells (CTCs) arise from primary or secondary tumors and enter the bloodstream by active passive intravasation. Given low number of CTCs, enrichment is necessary for detection. Filtration methods are based on selection CTCs size using a filter with 6.5 to 8 µm pores. After coloration, collected evaluated according morphological criteria. Immunophenotyping fluorescence in situ hybridization techniques may be used. Selected can also cultivated vitro provide more material. Analysis genomic mutations difficult because it requires adapted due limited DNA materials. Filtration-selected have shown prognostic value many studies but multicentric validating trials mandatory strengthen this assessment. Other clinical applications promising such as follow-up, therapy response prediction diagnosis. Microfluidic emerging systems could optimize filtration-selected increasing accuracy.