Recombinant NFAT1 (NFATp) Is Regulated by Calcineurin in T Cells and Mediates Transcription of Several Cytokine Genes
作者： C Luo , E Burgeon , J A Carew , P G McCaffrey , T M Badalian
关键词: Interleukin 2 、 COS cells 、 Promoter 、 Molecular biology 、 NFATC Transcription Factors 、 STAT4 、 Jurkat cells 、 Biology 、 Transcription factor 、 NFAT
摘要: Transcription factors of the NFAT family play a key role in transcription cytokine genes and other during immune response. We have identified two new isoforms factor NFAT1 (previously termed NFATp) that are predominant expressed murine human T cells. When Jurkat cells, recombinant is regulated, as expected, by calmodulin-dependent phosphatasecalcineurin,anditsfunctionisinhibitedbytheimmunosuppressiveagentcyclosporinA(CsA).Transactivation cells requires dual stimulation with ionomycin phorbol 12-myristate 13-acetate; this activity potentiated coexpression constitutively active calcineurin inhibited CsA. Immunocytochemical analysis indicates localizes cytoplasm transiently transfected translocates into nucleus CsA-sensitive manner following stimulation. COS however, capable transactivation, but it not regulated correctly: its subcellular localization transcriptional function affected 13-acetate. Recombinant can mediate interleukin-2, interleukin-4, tumor necrosis alpha, granulocyte-macrophage colonystimulating promoters suggesting contributes to these The nuclear activated (NFAT) was originallyidentifiedinTcellsasanessentialtranscriptionfactorfor
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