CARBOXYL-TERMINAL 15-AMINO ACID SEQUENCE OF NFATX1 IS POSSIBLY CREATED BY TISSUE-SPECIFIC SPLICING AND IS ESSENTIAL FOR TRANSACTIVATION ACTIVITY IN T CELLS

摘要: NFAT regulates transcription of a number cytokine and other immunoregulatory genes. We have isolated NFATx, which is one four members the family factors preferentially expressed in thymus peripheral blood leukocytes, an isoform NFATx1. Here we provide evidence showing that 15 amino acids carboxyl-terminal end NFATx1 are required for its maximum transactivation activity Jurkat T cells. A fusion between these GAL4 DNA binding domain was capable transactivating reporters driven by site. Interestingly, this 15-amino acid sequence well conserved proteins, although sequences contiguous to regions much less conserved. also report three additional isoforms designated NFATx2, NFATx3, NFATx4. This altered tissue-specific alternative splicing newly NFATx isoforms, resulting lower predominantly leukocyte, while skeletal muscle primarily NFATx2. In cells, from site IL-2 promoter activated strongly but only weakly These data demonstrate major induction genes cells possibly through splicing.