Multiple NF-ATc Isoforms with Individual Transcriptional Properties Are Synthesized in T Lymphocytes
作者： Friederike Berberich-Siebelt , Edgar Serfling , Thomas Brabletz , Inna Inashkina , Andris Avots
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摘要: The transcription factor NF-ATc that controls gene expression in T lymphocytes and embryonic cardiac cells is expressed three prominent isoforms. This due to alternative splice/polyadenylation events lead the predominant synthesis of two long isoforms naive a shorter isoform effector cells. Whereas previously described NF-ATc/A contains relatively short C terminus, longer isoforms, B C, span extra C-terminal peptides 128 246 aa, respectively. We show here addition strong N-terminal trans-activation domain, TAD-A, which common all NF-ATc/C second TAD-B, its peptide. Various stimuli induce activity TAD-A also enhance but, unlike TAD-B remains unphosphorylated by protein from 12-O-tetradecanoyl 12-phorbol 13-acetate-stimulated peptide NF-ATc/B exerts suppressive transcriptional effect. These properties -C might be importance for regulation are predominantly synthesized.
researchgate.net 参考文章(36)
C. Randak, T. Brabletz, M. Hergenröther, I. Sobotta, E. Serfling, Cyclosporin A suppresses the expression of the interleukin 2 gene by inhibiting the binding of lymphocyte-specific factors to the IL-2 enhancer. The EMBO Journal. ,vol. 9, pp. 2529- 2536 ,(1990) , 10.1002/J.1460-2075.1990.TB07433.X
Naoko Arai, Esteban S. Masuda, Ryu Imamura, Toshiya Takano, Shin-ichiro Imai, Ken-ichi Arai, Yoshiyuki Naito, Tadahiro Fujino, CARBOXYL-TERMINAL 15-AMINO ACID SEQUENCE OF NFATX1 IS POSSIBLY CREATED BY TISSUE-SPECIFIC SPLICING AND IS ESSENTIAL FOR TRANSACTIVATION ACTIVITY IN T CELLS Journal of Immunology. ,vol. 161, pp. 3455- 3463 ,(1998)
Ann M. Ranger, Michael J. Grusby, Martin R. Hodge, Ellen M. Gravallese, Fabienne Charles de la Brousse, Tim Hoey, Craig Mickanin, H. Scott Baldwin, Laurie H. Glimcher, The transcription factor NF-ATc is essential for cardiac valve formation Nature. ,vol. 392, pp. 186- 190 ,(1998) , 10.1038/32426
José Luis de la Pompa, Luika A. Timmerman, Hiroaki Takimoto, Hiroki Yoshida, Andrew J. Elia, Enrique Samper, Julia Potter, Andrew Wakeham, Luc Marengere, B. Lowell Langille, Gerald R. Crabtree, Tak W. Mak, Role of the NF-ATc transcription factor in morphogenesis of cardiac valves and septum Nature. ,vol. 392, pp. 182- 186 ,(1998) , 10.1038/32419
J W Rooney, Y L Sun, L H Glimcher, T Hoey, Novel NFAT sites that mediate activation of the interleukin-2 promoter in response to T-cell receptor stimulation. Molecular and Cellular Biology. ,vol. 15, pp. 6299- 6310 ,(1995) , 10.1128/MCB.15.11.6299
C Luo, E Burgeon, J A Carew, P G McCaffrey, T M Badalian, W S Lane, P G Hogan, A Rao, Recombinant NFAT1 (NFATp) Is Regulated by Calcineurin in T Cells and Mediates Transcription of Several Cytokine Genes Molecular and Cellular Biology. ,vol. 16, pp. 3955- 3966 ,(1996) , 10.1128/MCB.16.7.3955
Valérie Gruart-Gouilleux, Peter Engels, Michael Sullivan, Characterization of the human interleukin-5 gene promoter: involvement of octamer binding sites in the gene promoter activity European Journal of Immunology. ,vol. 25, pp. 1431- 1435 ,(1995) , 10.1002/EJI.1830250544
Sergei Chuvpilo, Michael Zimmer, Andreas Kerstan, Judith Glöckner, Andris Avots, Cornelia Escher, Christian Fischer, Inna Inashkina, Eriks Jankevics, Friederike Berberich-Siebelt, Edgar Schmitt, Edgar Serfling, Alternative polyadenylation events contribute to the induction of NF-ATc in effector T cells. Immunity. ,vol. 10, pp. 261- 269 ,(1999) , 10.1016/S1074-7613(00)80026-6
Juli D Klemm, Chan R Beals, Gerald R Crabtree, Rapid targeting of nuclear proteins to the cytoplasm Current Biology. ,vol. 7, pp. 638- 644 ,(1997) , 10.1016/S0960-9822(06)00290-9
José Aramburu, Francisco García-Cózar, Anuradha Raghavan, Heidi Okamura, Anjana Rao, Patrick G Hogan, Selective Inhibition of NFAT Activation by a Peptide Spanning the Calcineurin Targeting Site of NFAT Molecular Cell. ,vol. 1, pp. 627- 637 ,(1998) , 10.1016/S1097-2765(00)80063-5