SPECTROPHOTOMETRIC DETERMINATION OF ISONIAZID IN PURE AND PHARMACEUTICAL FORMULATIONS USING VANILLIN

摘要: Tuberculosis cases have significantly increased within the past decade, especially among AIDS patients, hence the importance of isoniazid, a first line anti‐tubercular agent. This has prompted many investigators to develope methods for the rapid determination of isoniazid in pure form as well as in pharmaceutical formulations. A method is described for the determination of isoniazid, in pure form and pharmaceutical formulations. The based on coupling isoniazid vanillin an ethanolic hydrochloric acid medium spectrophotometric determination at the absorption maximum (405nm). A yellow coloured hydrazone was formed. Beer’s law was obeyed in the concentration range of 1‐12μg/ml at 405nm. The proposed method was applied in the analysis of commercially purchased brands of isoniazid tablets and showed good accuracy precision. Excipients used formulation showed no interference analysis. The method offers the advantages of rapidity, simplicity and sensitivity and low cost and can be easily applied to resource‐poor settings without the need for expensive instrumentation and reagents.