Phosphorylation of Vasodilator-Stimulated Phosphoprotein Prevents Platelet-Neutrophil Complex Formation and Dampens Myocardial Ischemia-Reperfusion Injury

摘要: Background—Recent work has suggested that the formation of platelet-neutrophil complexes (PNCs) aggravates severity inflammatory tissue injury. Given importance vasodilator-stimulated phosphoprotein (VASP) for platelet function, we pursued role VASP on PNCs and its impact extent myocardial ischemia-reperfusion (IR) Methods Results—In initial in vitro studies found neutrophils facilitated movement platelets across endothelial monolayers. Phosphorylation reduced transendothelial PNCs. During IR injury, VASP−/− animals demonstrated intravascular presence within ischemic tissue. This was associated with Studies using transfer bone marrow chimeric showed hematopoietic expression crucial myoca...