UNC-40/DCC, SAX-3/Robo, and VAB-1/Eph Polarize F-Actin during Embryonic Morphogenesis by Regulating the WAVE/SCAR Actin Nucleation Complex
作者： Yelena Y. Bernadskaya , Andre Wallace , Jillian Nguyen , William A. Mohler , Martha C. Soto
DOI: 10.1371/JOURNAL.PGEN.1002863
关键词: Cell biology 、 Netrin 、 SCAR complex 、 Biology 、 Actin cytoskeleton 、 Erythropoietin-producing hepatocellular (Eph) receptor 、 Ephrin 、 Filamentous actin 、 Embryonic morphogenesis 、 Actin nucleation 、 Genetics(clinical) 、 Genetics 、 Cancer research 、 Ecology, Evolution, Behavior and Systematics 、 Molecular biology
摘要: Many cells in a developing embryo, including neurons and their axons growth cones, must integrate multiple guidance cues to undergo directed migration. The UNC-6/netrin, SLT-1/slit, VAB-2/Ephrin cues, receptors, UNC-40/DCC, SAX-3/Robo, VAB-1/Eph, are known be major regulators of cellular One important area research is identifying the molecules that interpret this information downstream receptors reorganize actin cytoskeleton. However, how regulate cytoskeleton not well understood. We report here VAB-1/Eph differentially abundance subcellular localization WAVE/SCAR nucleation complex its activator, Rac1/CED-10, Caenorhabditis elegans embryonic epidermis. Loss any these three pathways results embryos fail morphogenesis. Similar defects epidermal enclosure have been observed when CED-10/Rac1 or missing during development C. elegans. Genetic molecular experiments demonstrate fact, axonal proteins levels membrane enrichment Live imaging filamentous (F-actin) absence individual shows high F-actin essential for polarized cell migrations, but properly distribution essential. These suggest proper recruitment activation by signals at plasma result permits movements can distinct changes
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