Agrobacterium-mediated transformation of African violet
作者： Antonio Mercuri , Laura De Benedetti , Gianluca Burchi , Tito Schiva
关键词: Rhizobiaceae 、 Southern blot 、 Gene 、 Agrobacterium 、 Agrobacterium tumefaciens 、 Genetics 、 Petiole (botany) 、 Plasmid 、 Transformation (genetics) 、 Biology
摘要: A method for genetic transformation of Saintpaulia ionantha by co-cultivation in vitro-grown leaves and petioles with Agrobacterium tumefaciens is described. Two bacterial strains, EHA105 A281 both harbouring the binary plasmid pKIWI105 carrying genes uidA nptII, were used experiments. Regenerants not obtained using disarmed strain EHA105. The oncogenic resulted efficient transient stable expression transferred traits petiole explants only. After regeneration, integration transgenes plant genome was confirmed PCR analysis Southern hybridization.
cabdirect.org
springer.com
sci-hub.se 参考文章(18)
L. Semeria, B. Ruffoni, M. Rabaglio, A. Genga, A. M. Vaira, G. P. Accotto, A. Allavena, Genetic transformation ofEustoma grandiflorum byAgrobacterium tumefaciens Plant Cell Tissue and Organ Culture. ,vol. 47, pp. 67- 72 ,(1996) , 10.1007/BF02318967
Chin-Yi Lu, Greg Nugent, Terese Wardley-Richardson, Stephen F. Chandler, Richard Young, Michael J. Dalling, Agrobacterium-Mediated Transformation of Carnation (Dianthus caryophyllus L.) Nature Biotechnology. ,vol. 9, pp. 864- 868 ,(1991) , 10.1038/NBT0991-864
Christian Schöpke, Nigel Taylor, Rosa Cárcamo, N'Da Koffi Konan, Philippe Marmey, Graham G. Henshaw, Roger N. Beachy, Claude Fauquet, Regeneration of transgenic cassava plants (Manihot esculenta Crantz) from microbombarded embryogenic suspension cultures Nature Biotechnology. ,vol. 14, pp. 731- 735 ,(1996) , 10.1038/NBT0696-731
Richard A. Jefferson, Assaying chimeric genes in plants: The GUS gene fusion system Plant Molecular Biology Reporter. ,vol. 5, pp. 387- 405 ,(1987) , 10.1007/BF02667740
Ryutaro Aida, Michio Shibata, Transformation of Kalanchoe blossfeldiana mediated by Agrobacterium tumefaciens and transgene silencing Plant Science. ,vol. 121, pp. 175- 185 ,(1996) , 10.1016/S0168-9452(96)04524-4
AM Vaira, L Semeria, S Crespi, V Lisa, A Allavena, GP Accotto, Resistance toTospoviruses inNicotiana benthamianaTransformed with the N Gene of Tomato Spotted Wilt Virus: Correlation Between Transgene Expression and Protection In Primary Transformants Molecular Plant-Microbe Interactions. ,vol. 8, pp. 66- 73 ,(1995) , 10.1094/MPMI-8-0066
Xiu-Qing Li, Chang-Nong Liu, Steven W. Ritchie, Jian-ying Peng, Stanton B. Gelvin, Thomas K. Hodges, Factors influencing Agrobacterium-mediated transient expression of gusA in rice Plant Molecular Biology. ,vol. 20, pp. 1037- 1048 ,(1992) , 10.1007/BF00028891
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
SusanE. Ledger, SimonC. Deroles, NigelK. Given, Regeneration and Agrobacterium-mediated transformation of chrysanthemum Plant Cell Reports. ,vol. 10, pp. 195- 199 ,(1991) , 10.1007/BF00234294
Bart-Jan Janssen, Richard C. Gardner, Localized transient expression of GUS in leaf discs following cocultivation with Agrobacterium Plant Molecular Biology. ,vol. 14, pp. 61- 72 ,(1990) , 10.1007/BF00015655