Identification and characterization of two zebrafish Twik related potassium channels, Kcnk2a and Kcnk2b

摘要: KCNK2 is a 2 pore domain potassium channel involved in maintaining cellular membrane resting potentials. Although regarded as mechanosensitive ion channel, it can also be gated chemically. Previous research indicates that expression particularly enriched neuronal and cardiac tissues. In this respect, plays an important role neuroprotection has been linked to arrhythmias. subsequently become attractive pharmacologic target for developing preventative/curative strategies neuro/cardio pathophysiological conditions. Zebrafish represent vivo model rapidly analysing pharmacological compounds. We therefore sought identify characterise zebrafish kcnk2 allow system incorporated into therapeutic research. Our data possess two orthologs, kcnk2a kcnk2b. Electrophysiological analysis of both Kcnk2 orthologs shows that, like their human counterparts, they are activated by different physiological stimuli such mechanical stretch, polyunsaturated fatty acids intracellular acidification. Furthermore, channels inhibited the inhibitory peptide spadin. Taken together, our results demonstrate Kcnk2a Kcnk2b share similar biophysiological properties indicate will be a useful targeting strategies.