Noggin and chordin have distinct activities in promoting lineage commitment of mouse embryonic stem (ES) cells.
作者： Theresa E. Gratsch , K.Sue O'Shea
关键词: Embryonic stem cell 、 Neuroepithelial cell 、 Stem cell marker 、 Transfection 、 Molecular biology 、 Biology 、 Progenitor cell 、 Cell signaling 、 Chordin 、 Noggin
摘要: To examine the role of secreted signaling molecules and neurogenic genes in early development, we have developed a culture system for controlled differentiation mouse embryonic stem (ES) cells. In current investigation, two earliest identified BMP antagonists/neural-inducing factors, noggin chordin, were expressed pluripotent ES Neurons present as 24 h following transfection cells with pCS2/noggin expression plasmid, peaking at 72 h. With neuronal differentiation, cell marker down-regulated neural determination expressed. Coculture experiments exposure to noggin-conditioned medium produced similar control cells, while addition BMP-4 expressants strikingly inhibited differentiation. Transfection pCS2/chordin vector or chordin-conditioned more complex pattern differentiation; formed neurons, mesenchymal well N-CAM-positive, nestin-positive neuroepithelial progenitors. These data suggest that, consistent their different fields, chordin may play distinct roles patterning embryo.
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