Increased Cytosine DNA-Methyltransferase Activity During Colon Cancer Progression

摘要: BACKGROUND Molecular changes during progressive stages of colon cancer and other human tumors commonly involve altered regulation DNA methylation. These include overall genomic hypomethylation, regional hypermethylation, increased levels messenger RNA (mRNA) for cytosine DNA-methyltransferase (DNA-MTase), the enzyme that catalyzes methylation at CpG (cytosine-phospho-guanine) sites. This increase in DNA-MTase transcripts (mRNA), if accompanied by activity, could play a role abnormal patterns appear early tumor progression. PURPOSE We sought to determine whether mRNA progression are associated with cellular enzymatic activity. METHODS adapted microassay used it measure activity carcinoma mucosa normal control subjects patients or familial adenomatous polyposis (FAP), which is risk factor cancer. Steady-state gene were measured reverse transcriptase polymerase chain reaction assay. To compare levels, we determined both variables simultaneously one specimen, its adjacent mucosa, patient compared values. RESULTS Compared from subjects, was elevated 1.4-fold FAP 1.6-fold uninvolved cancer, 5.4-fold specimens. All these differences statistically significant. Fourteen 15 samples 47% had values higher than highest value mucosa. In who benign polyp malignant adenocarcinoma, increasing observed each stage CONCLUSION results demonstrate an capacity accompanies IMPLICATION Further studies needed this plays establishing seen malignancies.