Quantitative Analysis of the Expression and Distribution of Calcium Channel α 1 Subunit mRNA in the Atria and Ventricles of the Rat Heart

摘要: Abstract Two distinct calcium currents are present in mammalian cardiac myocytes. Utilizing quantitative RT-PCR methods, we have analysed the expression patterns and abundance of four channel α 1 subunit mRNAs different regions rat heart compared them to known density recorded from atria. Our results show that Ca V 1.2 is most abundant transcripts heart. The message more ventricle than atria does not vary as a function developmental age. 2.3, 3.1 3.2 10–100 times less 1.2. Interestingly, expressed both ventricle. atrial mRNA change significantly during development remains high older animals. In contrast, levels embryonic tissue at 3- 4-weeks postnatal but become undetectable 5 weeks. Expression 2.3 highest then declines gradually. We previously documented LVA current within 4–5 weeks after birth gradually reaching 30% its maximal value 12–14 complex relationship between suggests their contribution may