Developmental regulation of the L-type calcium channel α1C subunit expression in heart
作者： Lei Leu , Donald O'Hara , Steven Cala , Indu Poornima , Ronald Hines
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摘要: We used Northern analyses, RNase protection assays and immunoblot analyses to examine the relationship among developmental age of heart, abundance mRNA L-type calcium channel α1Csubunit protein, establish size native protein in heart. analysis, assays, immunoblots were study RNA from rat heart various ages. In fetal adult ventricles there was a predominant 8.3-kb transcript for α1C subunit with no change during development. demonstrated 2-fold increase DHP receptor message postnatal Immunoblots identified 240 kD corresponding predicted molecular mass full length subunit. No observed at any stage evidence truncated isoform. There an approximate ventricular homogenates Thus, developing alterations properties development appear result neither alternative splicing that produces smaller nor expression but least part transcriptionally-regulated kDa polypeptide.
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F C Chang, M M Hosey, Dihydropyridine and phenylalkylamine receptors associated with cardiac and skeletal muscle calcium channels are structurally different. Journal of Biological Chemistry. ,vol. 263, pp. 18929- 18937 ,(1988) , 10.1016/S0021-9258(18)37371-X
J.W. Hell, C.T. Yokoyama, S.T. Wong, C Warner, T.P. Snutch, W.A. Catterall, Differential phosphorylation of two size forms of the neuronal class C L-type calcium channel alpha 1 subunit. Journal of Biological Chemistry. ,vol. 268, pp. 19451- 19457 ,(1993) , 10.1016/S0021-9258(19)36536-6
X. Wei, A. Neely, A.E. Lacerda, R. Olcese, E. Stefani, E. Perez-Reyes, L. Birnbaumer, Modification of Ca2+ channel activity by deletions at the carboxyl terminus of the cardiac alpha 1 subunit. Journal of Biological Chemistry. ,vol. 269, pp. 1635- 1640 ,(1994) , 10.1016/S0021-9258(17)42074-6
Amy J. Davidoff, Tiina M. Maki, Øyvind Ellingsen, James D. Marsh, Expression of Calcium Channels in Adult Cardiac Myocytes is Regulated by Calcium Journal of Molecular and Cellular Cardiology. ,vol. 29, pp. 1791- 1803 ,(1997) , 10.1006/JMCC.1997.0406
Nikolai M. Soldatov, Genomic structure of human L-type Ca2+ channel. Genomics. ,vol. 22, pp. 77- 87 ,(1994) , 10.1006/GENO.1994.1347
John W. Schmidt, William A. Catterall, Biosynthesis and processing of the α subunit of the voltage-sensitive sodium channel in rat brain neurons Cell. ,vol. 46, pp. 437- 445 ,(1986) , 10.1016/0092-8674(86)90664-1
N Tohse, H Masuda, N Sperelakis, Novel isoform of Ca2+ channel in rat fetal cardiomyocytes. The Journal of Physiology. ,vol. 451, pp. 295- 306 ,(1992) , 10.1113/JPHYSIOL.1992.SP019165
Jörg Mitterdorfer, Monika Froschmayr, Manfred Grabner, Fabian F. Moebius, Hartmut Glossmann, Jörg Striessnig, Identification of PK-A phosphorylation sites in the carboxyl terminus of L-type calcium channel alpha 1 subunits. Biochemistry. ,vol. 35, pp. 9400- 9406 ,(1996) , 10.1021/BI960683O
Akira Yoshida, Masami Takahashi, Seiichiro Nishimura, Hiroshi Takeshima, Shinichiro Kokubun, Cyclic AMP-dependent phosphorylation and regulation of the cardiac dihydropyridine-sensitive Ca channel FEBS Letters. ,vol. 309, pp. 343- 349 ,(1992) , 10.1016/0014-5793(92)80804-P
R. J. Diebold, W. J. Koch, P. T. Ellinor, J. J. Wang, M. Muthuchamy, D. F. Wieczorek, A. Schwartz, Mutually exclusive exon splicing of the cardiac calcium channel alpha 1 subunit gene generates developmentally regulated isoforms in the rat heart. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 1497- 1501 ,(1992) , 10.1073/PNAS.89.4.1497