Expression cloning of a cDNA encoding the type II regulatory subunit of the cAMP-dependent protein kinase.
作者： Brian A. Hemmings , Manuela Schwarz , S. Rao Adavani , David A. Jans
DOI: 10.1016/0014-5793(86)81114-0
关键词: Protein kinase A 、 Peptide sequence 、 cDNA library 、 Expression cloning 、 Sequence analysis 、 Complementary DNA 、 Molecular cloning 、 Rapid amplification of cDNA ends 、 Biology 、 Molecular biology
摘要: We report here the isolation and sequence of a cDNA for type II regulatory subunit cAMP-dependent protein kinase (cAMP-PK) from λgt-11 library derived porcine epithelial cell line (LLC-PK1). The was detected by immunological screening using an affinity purified polyclonal antibody bovine RII. DNA analysis 467 bp EcoRI insert confirmed identity clone, because deduced amino acid corresponded to published RΠ protein. Northern total RNA LLC-PK1 cells indicated single mRNA species about 6.0 kb, probably copy gene.
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