Cultured hematopoietic stem cells and method for expansion and analysis thereof

作者: Harvey Lodish , Chengcheng Zhang

DOI:

关键词: Endothelial stem cellInduced stem cellsChemistryHaematopoiesisStem cell factorThrombopoietinFibroblast growth factorStem cellMolecular biologyGrowth factorCell biology

摘要: Hematopoietic stem cells and methods for ex vivo expansion of hematopoietic are provided. The comprise culturing the in a media containing an effective amount insulin-like growth factor(IGF), fibroblast factor (FGF), thrombopoietin (TPO), cell (SCF), under conditions sufficient said cells. Methods identifying expanded hematopoeitc kits also

参考文章(73)
R. E. Ploemacher, E. A. W. Blokland, S. Neben, D. A. Breems, Frequency analysis of human primitive haematopoietic stem cell subsets using a cobblestone area forming cell assay. Leukemia. ,vol. 8, pp. 1095- 1104 ,(1994)
Amy H. Yin, Sheri Miraglia, Esmail D. Zanjani, Graca Almeida-Porada, Makio Ogawa, Anne G. Leary, Johanna Olweus, John Kearney, David W. Buck, AC133, a Novel Marker for Human Hematopoietic Stem and Progenitor Cells Blood. ,vol. 90, pp. 5002- 5012 ,(1997) , 10.1182/BLOOD.V90.12.5002
Kateri A. Moore, Hideo Ema, Ihor R. Lemischka, In vitro maintenance of highly purified, transplantable hematopoietic stem cells. Blood. ,vol. 89, pp. 4337- 4347 ,(1997) , 10.1182/BLOOD.V89.12.4337
MG Cipolleschi, P Dello Sbarba, M Olivotto, The role of hypoxia in the maintenance of hematopoietic stem cells Blood. ,vol. 82, pp. 2031- 2037 ,(1993) , 10.1182/BLOOD.V82.7.2031.2031
Qun Shi, Shahin Rafii, Moses Hong-De Wu, Errol S. Wijelath, Cong Yu, Atsushi Ishida, Yuji Fujita, Sudesh Kothari, Robert Mohle, Lester R. Sauvage, Malcom A.S. Moore, Rainer F. Storb, William P. Hammond, Evidence for Circulating Bone Marrow-Derived Endothelial Cells Blood. ,vol. 92, pp. 362- 367 ,(1998) , 10.1182/BLOOD.V92.2.362
L Murray, B Chen, A Galy, S Chen, R Tushinski, N Uchida, R Negrin, G Tricot, S Jagannath, D Vesole, Enrichment of human hematopoietic stem cell activity in the CD34+Thy- 1+Lin- subpopulation from mobilized peripheral blood Blood. ,vol. 85, pp. 368- 378 ,(1995) , 10.1182/BLOOD.V85.2.368.368
Takashi Sato, Joseph H. Laver, Makio Ogawa, Reversible expression of CD34 by murine hematopoietic stem cells. Blood. ,vol. 94, pp. 2548- 2554 ,(1999) , 10.1182/BLOOD.V94.8.2548.420K38_2548_2554
Tzu-Bou Hsieh, Chi-Hsien Liu, I-Ming Chu, Shiaw-Min Hwang, Chao-Ling Yao, Optimizing culture medium for CD34 hematopoietic cell expansion 美國. ,(2004)