Hairpin primer amplification

作者: Kenneth B. Beckman , Ricardo Mancebo

DOI:

关键词: ChemistryNucleic acidMolecular biologyAsymmetric PCRPrimer (molecular biology)

摘要: Methods for amplifying nucleic acids (e.g., symmetric and asymmetric PCR methods) using a combination of linear hairpin primers to enhance specificity are provided. The primer can be fluorogenic primer, which facilitates detection acid target. Attendant compositions, systems, devices kits also

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