作者: Junko F. Stevens , Danny H. Lee , Shoulian Dong
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摘要: The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence undesired amplification products, sometimes referred as secondary products or spurious side-products. enzyme inhibitors disclosed herein comprise a nucleotide sequence at least one quencher. Complexes comprising an inhibitor associated with enzyme, wherein enzymatic activity of the is inhibited, also provided. Methods acid disclosed, methods fluorescence. Kits expediting performance certain