Compositions, methods, and kits for amplifying nucleic acids

作者: Junko F. Stevens , Danny H. Lee , Shoulian Dong

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摘要: The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence undesired amplification products, sometimes referred as secondary products or spurious side-products. enzyme inhibitors disclosed herein comprise a nucleotide sequence at least one quencher. Complexes comprising an inhibitor associated with enzyme, wherein enzymatic activity of the is inhibited, also provided. Methods acid disclosed, methods fluorescence. Kits expediting performance certain

参考文章(72)
O. Yu. Yakimovich, Ya. I. Alekseev, A. V. Maksimenko, O. L. Voronina, V. G. Lunin, Influence of DNA aptamer structure on the specificity of binding to Taq DNA polymerase. Biochemistry. ,vol. 68, pp. 228- 235 ,(2003) , 10.1023/A:1022609714768
Kenneth B. Beckman, Ricardo Mancebo, Hairpin primer amplification ,(2004)
Peter E Nielsen, None, Peptide nucleic acids : protocols and applications Horizon Bioscience. ,(2004)
Z. Shabarova, A. Bogdanov, Advanced Organic Chemistry of Nucleic Acids ,(1994)
Ralph Rapley, The nucleic acid protocols : handbook Humana Press. ,(2000)
G. Michael Blackburn, M. J. Gait, Nucleic Acids in Chemistry and Biology ,(1989)
John L. Daiss, David J. Sharkey, Kenneth G. Christy, Theodore W. Esders, Edward R. Scalice, Thermostable polymerase specific antibody-containing DNA amplification composition and kit ,(1994)
Katarzyna Bebenek, Thomas A. Kunkel, Functions of DNA Polymerases Advances in Protein Chemistry. ,vol. 69, pp. 137- 165 ,(2004) , 10.1016/S0065-3233(04)69005-X
Sergey G Lokhov, Eugeny A Lukhtanov, Nicolaas M J Vermeulen, Negatively charged minor groove binders ,(2003)