Multidrug resistance increment in a human colon carcinoma cell line by colchicine

摘要: The most important mechanism in drug resistance is the multidrug (MDR) phenomenon. It possible to select MDR cells by vitro exposure cytotoxic agents. due hyperexpression of P-glycoprotein (PGp) that take drugs out from cells. In this study, a colchicine resistant subline (HCA-2/1cch) was selected human colon adenocarcinoma after short period exposure, as an model selection. These showed cross-resistance other drugs, which were not present medium during relative 3.32 for colchicine, 3.15 vinblastine, 2.62 vincristine and 5.22 mitomycin C. levels assayed flow cytometry. found significant increase 2.35 1.59 had occurred peak mean channel fluorescence, respectively, indicating increment expression relation parental line. Moreover, verapamil (10 μg/ml) produced partial reversion resistance. sensitisation rates 7.41 1.25 2.36 1.17 data obtained suggest weeks) dose (0.5 sufficient produce This could be higher level P-Gp expression.