P-glycoprotein, glutathione and glutathione S-transferase increase in a colon carcinoma cell line by colchicine.

摘要: The acquisition of resistance to anticancer agents used in chemotherapy is the main cause treatment failure malignant disorders, provoking tumours become resistant during treatment, although they initially respond it. multidrug (MDR) mechanism tumour cells expression P-glycoprotein (P-gly), that acts as an ATP-dependent active efflux pump chemotherapeutic agents. Furthermore, increased detoxification compounds mediated by high levels glutathione (GSH) and S-transferase (GST), has been found cells. We developed a study aiming evaluate evolution drug markers cells: P-gly, GSH GST, colchicine, for purpose studying adaptation process its contribution MDR phenomenon. A human colon adenocarcinoma cell line was exposed colchicine 82 days, being GST activity evaluated flow cytometry, spectrofluorimetry spectrophotometry, exposure time. P-gly gradually reaching 2.35 3.21 fold each. On day 82, 1.84 at end period. Moreover, increment cross-resistance obtained ranges from 2.62 5.22 vinblastine, vincristine mitomycin C. increments could probably contribute phenomenon this line.