Cloning, genomic organisation and mRNA expression of a pectin lyase gene from a mutant strain of Penicillium occitanis.
作者： Hèla Trigui-Lahiani , Ali Gargouri
DOI: 10.1016/J.GENE.2006.09.022
关键词: Gene 、 Complementary DNA 、 Homology (biology) 、 Biology 、 Genetics 、 Aspergillus niger 、 Penicillium occitanis 、 Nucleic acid sequence 、 Consensus sequence 、 Pectin lyase
摘要: The regulatory cis elements of fungal pectinases are well studied in Aspergillus genera but little is known other species. A genomic bank from Penicillium occitanis fungus constructed and screened by previously isolated cDNA probe a pectin lyase. From several clones, the nucleotide sequence lyase gene was completed led to identification introns promoter-terminator regions. streaking future found pnl P. occitanis: it exhibits highest homology with pnlA niger positions its 4 completely identical that A. pnlB gene. In addition determination transcription start site, promoter analysed. It showed conservation consensus sequences -CreA, Hap2-3-4, PacC ...-, existence particular -CCTGA- which similar already be specific pectinolytic Aspergillus, CCCTGA. This result suggests corresponding trans-acting factor should same as Aspergillus.
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