Multiple Kinase Pathways Regulate Voltage-Dependent Ca2+ Influx and Migration in Oligodendrocyte Precursor Cells

摘要: It is becoming increasingly clear that voltage-operated Ca(2+) channels (VOCCs) play a fundamental role in the development of oligodendrocyte progenitor cells (OPCs). Because direct phosphorylation by different kinases one most important mechanisms involved VOCC modulation, aim this study was to evaluate participation serine-threonine and tyrosine (TKs) on influx mediated VOCCs OPCs. Calcium imaging revealed OPCs exhibited after plasma membrane depolarization via L-type VOCCs. Furthermore, VOCC-mediated declined with OPC differentiation, indicating are developmentally regulated PKC activation significantly increased activity OPCs, whereas PKA produced opposite effect. The results also indicated morphological changes induced were partially Our data clearly suggest TKs exert an activating influence function using PDGF response as model probe TK receptors (TKr) uptake, we found TKr potentiated depolarization. Interestingly, modulation appeared be essential for enhancement migration rate, because cell motility completely blocked antagonists, well inhibitors, assays. present strongly demonstrates TKrs enhance has inhibitory These modulate uptake participate process extension migration.