The HCF136 protein is essential for assembly of the photosystem II reaction center in Arabidopsis thaliana.
作者： Henning Plücken , Bernd Müller , Dina Grohmann , Peter Westhoff , Lutz A Eichacker
DOI: 10.1016/S0014-5793(02)03634-7
关键词: Photosystem I 、 Photoinhibition 、 P700 、 Biology 、 Photosystem II 、 Light-harvesting complexes of green plants 、 Cytochrome b559 、 Biophysics 、 Cytochrome b6f complex 、 Photosystem 、 Biochemistry
摘要: Hcf136 encodes a hydrophilic protein localized in the lumen of stroma thylakoids. Its mutational inactivation Arabidopsis thaliana results photosystem II (PHII)-less phenotype. Under standard illumination, PSII is not detectable and amount I (PSI) reduced, which implies that HCF136p may be required for biogenesis general. However, at low light, comparison mutants with defects PSII, PSI, cytochrome b6f complex reveals regulates selectively PSII. We demonstrate by vivo radiolabeling hcf136 reaction center (RC) blocked. Gel blot analysis affinity chromatography solubilized thylakoid membranes suggest associates precomplex containing least D2 b559. conclude essential assembly RC discuss its function as chaperone-like factor.
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