Speciation of chromium using chronoamperometric biosensors based on screen-printed electrodes.
作者： Ana Calvo-Pérez , Olga Domínguez-Renedo , MAsunción Alonso-Lomillo , MJulia Arcos-Martínez
DOI: 10.1016/J.ACA.2014.05.006
关键词: Tetrathiafulvalene 、 Glucose oxidase 、 Chemistry 、 Tap water 、 Chromium 、 Inorganic chemistry 、 Tyrosinase 、 Biosensor 、 Electrode 、 Ferricyanide
摘要: Abstract Chronoamperometric assays based on tyrosinase and glucose oxidase (GOx) inactivation have been developed for the monitoring of Cr(III) Cr(VI). Tyrosinase was immobilized by crosslinking screen-printed carbon electrodes (SPCEs) containing tetrathiafulvalene (TTF) as electron transfer mediator. The tyrosinase/SPC TTF E response to pyrocatechol is inhibited Cr(III). This process, that not affected Cr(VI), allows determination with a capability detection 2.0 ± 0.2 μM reproducibility 5.5%. GOx modified platinised (SPC Pt Es) were selective Cr(VI) using ferricyanide redox biosensor able discriminate two different oxidation states chromium being reject detect toxic species towards decreases presence 90.5 ± 7.6 nM 6.2%. A bipotentiostatic chronoamperometric finally GOx/SPC connected in array mode simultaneous spiked tap water waste from tannery factory samples.
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