Hyperglycemia induces apoptosis in pre-implantation embryos through cell death effector pathways

摘要: Although perinatal mortality rates have improved for pregnant diabetic women because of insulin therapy and tight metabolic control, infants diabetics still experience significantly higher congenital malformations spontaneous miscarriages compared with those non-diabetic women. Our results here indicate that hyperglycemic conditions, either in vivo or vitro, modulate the expression an apoptosis regulatory gene as early pre-implantation blastocyst stage mouse. Apoptosis mammalian is a normal process, thought to protect embryo by eliminating abnormal cells. Here we demonstrate Bax, Bcl-2-like protein, increased at presence high concentrations glucose, these changes correlate morphologically DNA fragmentation. Expression Bax caspase are necessary this vitro glucose-induced apoptotic event, ceramide involved mediating embryotoxic effect glucose. We also show cellular can be prevented treating mice before immediately after conception. These findings emphasize importance glycemic control earliest stages