Fat Grafting in Burn Scar Alleviates Neuropathic Pain via Anti-Inflammation Effect in Scar and Spinal Cord
作者： Shu-Hung Huang , Sheng-Hua Wu , Su-Shin Lee , Kao-Ping Chang , Chee-Yin Chai
DOI: 10.1371/JOURNAL.PONE.0137563
关键词: Skin grafting 、 Scars 、 Burn injury 、 Anesthesia 、 Neuralgia 、 Neuroinflammation 、 Spinal cord 、 Pathology 、 Neuropathic pain 、 Medicine 、 Transplantation
摘要: Burn-induced neuropathic pain is complex, and fat grafting has reportedly improved pain. However, the mechanism of in improving unclear. Previous investigations have found that neuroinflammation causes pain, anti-inflammatory targeting may provide potential therapeutic opportunities We hypothesized burn scars improves through anti-inflammation. scar was confirmed using a mechanical response test 4 weeks after injuries, autologous area performed simultaneously. After weeks, animals were sacrificed, specimens collected for inflammation test, including COX-2, iNOS, nNOS injured skin spinal cord dorsal horns immunohistochemistry Western assays. Furthermore, pro-inflammatory cytokines (IL-1 β TNF-α) collected. Double immunofluorescent staining images measuring p-IκB, p-NFκB, p-JNK, TUNEL as well blots AKT, Bax/Bcl-2 inflammatory process, apoptosis analyzed. Fat significantly reduced COX2, nNOS, iNOS horns, IL-1β TNF-α, compared with group. Moreover, regarding effect, cells decreased injury effective treating burn-induced alleviation ameliorated neuronal apoptosis.
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