Human α-Defensins HNPs-1, -2, and -3 in Renal Cell Carcinoma : Influences on Tumor Cell Proliferation

摘要: The α-defensins human neutrophil peptides (HNPs)-1, -2, and -3 have been described as cytotoxic with restricted expression in neutrophils some lymphocytes. In this study we report that HNPs-1, are also expressed renal cell carcinomas (RCCs). Several RCC lines were found to express mRNA well the specific of HNP-1, demonstrated by reverse transcriptase-polymerase chain reaction, mass spectrometric, flow cytometric analyses. At physiological concentrations stimulated proliferation selected vitro but at high for all tested. As lines, detected vivo malignant epithelial cells 31 tissues addition their expected presence neutrophils. most cases randomly, patchy immunostaining on surrounding was seen, six tumors higher grade malignancy tumor diffusely stained. Cellular necrosis observed association extensive patches -3, seemed be related α-defensins. findings suggest frequent peptide constituents a possible direct influence proliferation.