In Vivo Imaging of Amyloid-β Deposits in Mouse Brain With Multiphoton Microscopy

摘要: With the advent of transgenic mouse models expressing cortical amyloid pathology, potential to study its progression in an intact brain has been realized. Multiphoton microscopy provides a non-destructive means imaging with micron resolution up 500 microm deep into cortex. We detail surgical procedure and discuss multiphoton approach that allows for labeling chronic visualization amyloid-beta deposits through cranial window. The ability monitor these hallmarks Alzheimer's disease enables studies aimed at evaluating efficacy treatment prevention strategies.