γ-H2AX induced by linear alkylbenzene sulfonates is due to deoxyribonuclease-1 translocation to the nucleus via actin disruption.
作者： Xiaoxu Zhao , Tatsushi Toyooka , Toru Kubota , Guang Yang , Yuko Ibuki
DOI: 10.1016/J.MRFMMM.2015.04.006
关键词: DNA 、 DNA replication 、 Biophysics 、 Nucleus 、 EGTA 、 Molecular biology 、 DNA damage 、 Actin 、 Cell cycle 、 Biology 、 Phosphorylation
摘要: Phosphorylation of histone H2AX (γ-H2AX) occurs following formation DNA double strand breaks (DSBs). Other types damage also generate DSBs through replication and repair, leading to the production γ-H2AX. In present study, we demonstrated that linear alkylbenzene sulfonates (LAS), most widely used non-genotoxic anionic surfactants, could γ-H2AX via a novel pathway. Breast adenocarcinoma MCF-7 cells were treated with five kinds LAS alkyl chains ranging from 10 14 carbon units (C10-C14LAS). The generation subsequent increased in manner depended on number LAS. be generated non-cytotoxic doses was independent cell cycle, indicating non-apoptotic replication-independent DSBs. attenuated by EGTA ZnCl2, deoxyribonuclease-1 (DNase I) inhibitors, as well knockdown DNase I. weakened interaction between I actin, enhanced release dependent released treatment translocated nucleus, which attacked These results suggested LAS-induced attributed translocation nucleus disruption not damage.
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