Inhibition of deoxyribonuclease I by actin is to protect cells from premature cell death

摘要: Deoxyribonuclease I (Dnase1) is the major extracellular endonuclease. It secreted by digestive glands into alimentary tract and plasma, lacrimal fluid urine hepatocytes, renal proximal tubular cells, respectively. In many species activity of Dnase1 inhibited monomeric actin. However, biological significance this high affinity interaction unknown. We generated a mutant with extremely reduced actin binding capacity. EGFP-constructs wild-type were transfected MCF-7 breast cancer cells apoptosis or necrosis was induced staurosporine oxidative stress. During faster chromatin fragmentation occurred in Dnase1. When wt (wild-type)- mutated added to after induction necrosis, degradation presence Inclusion under these conditions wt- but not Thus, inhibition may serve as self-protection mechanism against premature DNA during cell damage.