Enhanced Detection of Viruses in Cell Cultures

摘要: Several viruses such as herpes simplex virus (HSV), respiratory syncytial (RSV), influenza virus, and cytomegalovirus (CMV) were able to infect cells cultured in vitro, but the time for recognition of cytopathic effects (CPE) produced by these agents ranged from a few days several weeks. In addition age cells, condition cell monolayers, specificity monoclonal antibodies immediate-early or early viral antigens, other important variables include incubation temperature, number shell vial cultures used per specimen, centrifugation, type specimen (urine, blood, bronchoalveolar lavage (BAL) fluid, tissue), quality fluorescence equipment, chemical pretreatment mono-layers, technical experience with assay subjectively evaluate specific results. comparisons have demonstrated that is sensitive (for HSV tract [adenoviruses, parainfluenza types 1, 2, 3, enterovirus, A B measles mumps RSV]) even more than CMV) recovery conventional tube cultures, which may require weeks CPE. The diagnostic laboratory always find use cultures; however, next level test performance clinical will be formatted automated extraction quantitation detection target nucleic acids. Amplified acids monitored real thermocycling instruments designed routine biosafety 2 laboratories.