Applicability of a Real-Time Quantitative PCR Assay for Diagnosis of Respiratory Syncytial Virus Infection in Immunocompromised Adults

摘要: Respiratory syncytial virus (RSV) accounts for the majority of respiratory infections, producing high mortality rates in immunocompromised patients with hematologic malignancies. The available methods rapid detection RSV by antigen or PCR either lack sensitivity, require complex laboratory manipulation, have not been evaluated this patient population. To assess applicability a TaqMan-based real-time technique A and B adults, we developed rapid, sensitive method that simultaneously detects can be applied routine diagnostics. specificity assay was assessed using panel reference strains other viruses RSV. Electron microscopy-counted stocks were used to develop quantitative format. Eleven copies viral RNA could detected strain Long, 14 9320, corresponding 50% tissue culture infective doses 0.86 0.34, respectively. on 411 combined nose throat swabs derived from adults without signs tract infection. diagnostic efficacy TaqMan determined clinical samples showed substantially more than combination conventional shell vial culture. None specimens illness found positive PCR.