Inhibition of oocyte growth factors in vivo modulates ovarian folliculogenesis in neonatal and immature mice.

摘要: Growth differentiation factor-9 (GDF9) and bone morphogenetic protein-15 (BMP15) are among the key regulators transmitting signaling between oocyte surrounding granulosa cells. Previously, it has been shown that a recombinant BMP type II receptor ectodomain-Fc fusion protein (BMPR2ecd-Fc) is able to inhibit actions of GDF9 BMP15 in vitro. Here, we have produced bioactive BMPR2ecd-Fc, which was injected i.p. into neonatal mice. Early folliculogenesis first studied by injecting mice five times with various doses BMPR2ecd-Fc during postnatal days 4-12. Folliculogenesis affected dose dependently, as evidenced decreased mitogenesis cells growing follicles. Furthermore, also noticed decrease number secondary tertiary follicles well an increase size. Electron microscopic analysis revealed ultrastructure primary not treatment. A second study conducted investigate whether longer treatment 12 injections 4-28 would folliculogenesis. Similar effects were observed two studies on early follicular developmental stages. However, long-term study, later stages blocked but rather increased numbers antral follicles, preovulatory corpora lutea found. We conclude potent modulator ovarian vivo, thus, valuable tool for studying physiology downstream oocyte-derived growth factors vivo.