Effects of jacalin and follicle-stimulating hormone on in vitro goat primordial follicle activation, survival and gene expression.
作者： Regislane P Ribeiro , Antonia MLR Portela , Anderson WB Silva , José JN Costa , José RS Passos
DOI: 10.1017/S0967199414000173
关键词:
摘要: This study aims to investigate the effects of jacalin and follicle-stimulating hormone (FSH) on activation survival goat primordial follicles, as well gene expression in cultured ovarian tissue. Ovarian fragments were for 6 days minimum essential medium (MEM) supplemented with (10, 25, 50 or 100 μg/ml - Experiment 1) MEM (50 μg/ml), FSH ng/ml) both (Experiment 2). Non-cultured tissues processed histological ultrastructural analysis. Cultured from 2 also stored evaluate BMP-15, KL (Kit ligand), c-kit, GDF-9 proliferating cell nuclear antigen (PCNA) by real-time polymerase chain reaction (PCR). The results 1 showed that, compared tissue that was control medium, presence increased percentages developing follicles viability. In 2, after days, higher normal observed FSH, both, but no synergistic interaction between observed. These substances had significant effect levels mRNA BMP-15 KL, significantly PCNA c-kit. On other hand, reduced GDF-9. conclusion, are able improve follicle culture. Furthermore, increases resulted lower expression.
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