Biochemical Production with Purified Cell-Free Systems

摘要: Abstract Bioprocesses offer a powerful set of opportunities for current chemical process innovation. Such conversions provide to employ intensification strategies and the right characteristics scale down harness power modular design, but strain engineering use microbial systems industrial biochemicals production remain unpredictable expensive. Purified cell-free technologies, in which complex enzyme reaction cascades are used catalyze bioconversions interest, can be designed mitigate these limitations. allow predictive kinetic modeling full control conductions, enabling dramatically increased design-built-test cycle iteration rate. When sufficiently robust engineered enzymes used, leverage harsh conditions, long-term recycling strategies, hybridization with catalysis improve value proposition production. Further research is required realize potential approaches address platform-limiting challenges around cofactor cost reuse.