A novel method for preparation of MNP@CS-tethered coenzyme for coupled oxidoreductase system.
作者： Guo Chen , Zhichao Wu , Yunhui Ma
DOI: 10.1016/J.JBIOTEC.2015.01.016
关键词: Glycerol-3-phosphate dehydrogenase 、 Benzyl alcohol 、 Alcohol dehydrogenase 、 NAD+ kinase 、 Stereochemistry 、 Acetaldehyde 、 Cofactor 、 Chemistry 、 Succinimide 、 Oxidoreductase
摘要: Abstract The immobilized cofactor NAD(H) is easily recovered from the reaction bulk, which essential for repeated use of in bioprocess catalyzed by NAD(H)-dependent oxidoreductase. Here, a magnetic nanoparticle platform was designed to immobilize both NADH and NAD + . design based on chitosan-coated nanoparticles (MNP@CS) activated EDC/NHS with aid azelaic acid as spacer. Interestingly, succinimide group at end spacer arm direct coupling carboxyl-terminal 6-amino adenine residue NAD(H). Our results indicated that 150 μmol 50 μmol effectively attached 1 g MNP@CS 25 °C 120 min prepared MNP@CS–NAD(H) showed good activity according benzyl alcohol acetaldehyde dehydrogenase.
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