Effect of molecular mobility on coupled enzymatic reactions involving cofactor regeneration using nanoparticle-attached enzymes

摘要: Cofactor-dependent multi-step enzymatic reactions generally require dynamic interactions among cofactor, enzyme and substrate molecules. Maintaining such molecular can be quite challenging especially when the catalysts are tethered to solid state supports for heterogeneous catalysis either biosynthesis or biosensing. The current work examines effects of pattern immobilization, which presumably impacts on surface supports, reaction kinetics a multienzymic system including glutamate dehydrogenase, glucose dehydrogenase cofactor NAD(H). Interestingly, particle collision due Brownian motion nanoparticles successfully enabled coupled involving regeneration cycle NAD(H) even enzymes were immobilized separately onto superparamagnetic (124 nm). impact was evident in that overall rate increased by over 100% applying moderate alternating magnetic field (500 Hz, 17 Gs), using additional spacers, both could improve mobility catalysts. We further observed integrated allowed placed vicinity same nanoparticles, enhance 1.8 fold. These results demonstrated feasibility manipulating catalyst components nanoscale fabrication efficient biosynthesis. (C) 2011 Elsevier B. V. All rights reserved.