Movement of a barley stripe mosaic virus chimera with a tobacco mosaic virus movement protein

摘要: Abstract The tobacco mosaic virus (TMV) 30K movement protein (MP) gene was inserted into a full-length cDNA clone of barley stripe (BSMV) RNAβ replacing the triple block (TGB). resulting recombinant ND-MPT genome, consisting infectious wt transcripts BSMV RNAs α and γ, together with hybrid transcript, inoculated onto test plants to study functional compatibility between TGB-adapted genetic system tobamovirus transport gene. infected leaves ofNicotiana benthamianaandChenopodium amaranticolor,which are common hosts for parental viruses; size, growth rate, morphology local lesions onC. amaranticolorwere influenced by foreign MP However, failed infect barley,N. tabacum(var. Samsun), andN. clevelandii,the selective hosts. Thus, TMV able functionally substitute TGB-coded MPs, i.e., functioned independently any other sequences. promoted cell-to-cell in host-dependent manner.