Identification of arabidopsis proteins that interact with the cauliflower mosaic virus (CaMV) movement protein.
作者： Zhong Huang , Vyacheslav M. Andrianov , Yu Han , Stephen H. Howell
关键词:
摘要: Gene I of cauliflower mosaic virus (CaMV) encodes a protein that is required for movement. The CaMV movement (MP) was used in yeast 2-hybrid system to screen an Arabidopsis cDNA library cDNAs encoding MP-interacting (MPI) proteins. Three different clones were found proteins (MPI1, -2 and -7) interact with the N-terminal third MP. interaction between MPI7 MP mutants correlated infectivity mutants. A non-infectious mutant, ER2A, two amino acid changes failed MPI7, while infectious second-site differed from ER2A by only single change, interacted system. encoded member large, but diverse gene family Arabidopsis. related sequence, size hydropathy profile mammalian (such as rat PRA1) described rab acceptor. expressed widely plants, transgenic plants MPI7:GFP fusion localized cytoplasm, concentrated punctate spots. In protoplasts transfected CFP:MP MPI7:YFP, colocalized some sites where MPI7:YFP expressed. At these sites, fluorescence resonance energy transfer (FRET) fluorophores observed indicating planta MPI7.
springer.com
sci-hub.se 参考文章(77)
J.G. Atabekov, Yu. L. Dorokhov, Plant Virus-Specific Transport Function And Resistance Of Plants To Viruses Advances in Virus Research. ,vol. 29, pp. 313- 364 ,(1984) , 10.1016/S0065-3527(08)60412-1
Paloma Más, Paul F. Devlin, Satchidananda Panda, Steve A. Kay, Functional interaction of phytochrome B and cryptochrome 2 Nature. ,vol. 408, pp. 207- 211 ,(2000) , 10.1038/35041583
R. A. Jorgensen, BOTANY: An RNA-Based Information Superhighway in Plants Science. ,vol. 279, pp. 1486- 1487 ,(1998) , 10.1126/SCIENCE.279.5356.1486
J Wellink, J W van Lent, J Verver, T Sijen, R W Goldbach, A van Kammen, The cowpea mosaic virus M RNA-encoded 48-kilodalton protein is responsible for induction of tubular structures in protoplasts. Journal of Virology. ,vol. 67, pp. 3660- 3664 ,(1993) , 10.1128/JVI.67.6.3660-3664.1993
A.G. SOLOVYEV, D.A. ZELENINA, E.I. SAVENKOV, V.Z. GRDZELISHVILI, S.YU. MOROZOV, D.-E. LESEMANN, E. MAISS, R. CASPER, J.G. ATABEKOV, Movement of a barley stripe mosaic virus chimera with a tobacco mosaic virus movement protein Virology. ,vol. 217, pp. 435- 441 ,(1996) , 10.1006/VIRO.1996.0137
E. Waigmann, W. J. Lucas, V. Citovsky, P. Zambryski, Direct functional assay for tobacco mosaic virus cell-to-cell movement protein and identification of a domain involved in increasing plasmodesmal permeability. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 91, pp. 1433- 1437 ,(1994) , 10.1073/PNAS.91.4.1433
RICHARD KORMELINK, DICK PETERS, JAN W.M. VAN LENT, ROB W. GOLDBACH, MARC M.H. STORMS, The nonstructural NSm protein of tomato spotted wilt virus induces tubular structures in plant and insect cells. Virology. ,vol. 214, pp. 485- 493 ,(1995) , 10.1006/VIRO.1995.0059
Amine O. Noueiry, William J. Lucas, Robert L. Gilbertson, Two proteins of a plant DNA virus coordinate nuclear and plasmodesmal transport Cell. ,vol. 76, pp. 925- 932 ,(1994) , 10.1016/0092-8674(94)90366-2
C.L. Thomas, C. Perbal, A.J. Maule, A Mutation of Cauliflower Mosaic Virus Gene I Interferes with Virus Movement but Not Virus Replication Virology. ,vol. 192, pp. 415- 421 ,(1993) , 10.1006/VIRO.1993.1056
J. van Lent, M. Storms, F. van der Meer, J. Wellink, R. Goldbach, Tubular structures involved in movement of cowpea mosaic virus are also formed in infected cowpea protoplasts. Journal of General Virology. ,vol. 72, pp. 2615- 2623 ,(1991) , 10.1099/0022-1317-72-11-2615