Multi-Omics Characterization of Inflammatory Bowel Disease-Induced Hyperplasia/Dysplasia in the Rag2-/-/Il10-/- Mouse Model.
作者： Qiyuan Han , Thomas J. Y. Kono , Charles G. Knutson , Nicola M. Parry , Christopher L. Seiler
DOI: 10.3390/IJMS22010364
关键词: Cancer research 、 Epigenetics 、 DNA methylation 、 Reduced representation bisulfite sequencing 、 Carcinogenesis 、 Helicobacter hepaticus 、 Biology 、 Transcriptome 、 Cancer 、 Differentially methylated regions
摘要: Epigenetic dysregulation is hypothesized to play a role in the observed association between inflammatory bowel disease (IBD) and colon tumor development. In present work, DNA methylome, hydroxymethylome, transcriptome analyses were conducted proximal tissues harvested from Helicobacter hepaticus (H. hepaticus)-infected murine model of IBD. Reduced representation bisulfite sequencing (RRBS) oxidative RRBS (oxRRBS) identified 1606 differentially methylated regions (DMR) 3011 hydroxymethylated (DhMR). These DMR/DhMR overlapped with genes that are associated gastrointestinal disease, cancer. RNA-seq revealed pronounced expression changes number inflammation Several including Duox2, Tgm2, Cdhr5, Hk2 exhibited both methylation/hydroxymethylation gene levels. Overall, our results suggest chronic triggers methylation hydroxymethylation patterns genome, altering key tumorigenesis potentially contributing initiation colorectal
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