Identification of piroplasm infection in questing ticks by RLB: a broad range extension of tick-borne piroplasm in China?
作者： Mirza Omar Abdallah , Qingli Niu , Peifa Yu , Guiquan Guan , Jifei Yang
DOI: 10.1007/S00436-016-4947-Z
关键词: Piroplasmida 、 Babesia bigemina 、 Theileria 、 Biology 、 Virology 、 Plasmid 、 Tick 、 Babesia 、 18S ribosomal RNA 、 Ribosomal RNA
摘要: Sensitive and specific diagnostic method for rapid simultaneous detection discrimination of the different species is needed an effective control piroplasmosis. Here, a reverse line blot (RLB) assay was developed piroplasm detection. A general pair primer based on 18S ribosomal RNA (rRNA) gene used to amplify V4 region rRNA gene. General probes 13 were cited from previous publications or designed according alignment sequences. For sensitivity test RLB assay, serially diluted plasmids access RLB. Four hundred fifty tick samples collected grass provinces China then detected. The result indicated that highly sensitive, detecting up 10(2) copies/μl recombinant plasmid DNA. Multiple piroplasms detected as single mixed infection species. Eight species, most which Theileria annulata (33/450, 7.3 %) Babesia sp. Xinjiang (30/450, 6.7 %), found infect with 89 in four species; no infections major, ovata, bigemina, sergenti, equi species-specific may have potential clinical application differentiation
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