Ovary co-culture improves embryo and green plant production in anther culture of Australian spring wheat ( Triticum aestivum L.)
作者： Sue Broughton
DOI: 10.1007/S11240-008-9432-7
关键词: Botany 、 Tissue culture 、 Horticulture 、 Embryo culture 、 Poaceae 、 Cultivar 、 Biology 、 Doubled haploidy 、 Stamen 、 Ovary (botany) 、 Plant physiology
摘要: A simple anther culture protocol for Australian spring wheat cultivars was developed using ovary co-culture. The inclusion of ovaries in the induction medium significantly increased production embryo-like structures (ELS), green and albino plants two tested. When five were added to medium, mean number ELS per spike from 7.6 50.1 0.6 8.9. addition 10 ovaries, however, did not further increase or plants. growth regulator combination 2,4-D KIN compared with IAA BA. There no significant differences numbers although fewer produced Eight additional screened either 5 medium. Green obtained nine varieties at frequencies ranging 0.3 33.0 spike. Regenerant maturity exhibited chromosome fertility rates different 15% 100%.
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