Protocol for wheat (Triticum aestivum L.) anther culture

摘要: After many years of different experiments around the world on haploid production methods have become available to breeders. In Szeged, in vitro androgenesis via anther culture is one most efficient systems homozygous line production. It can be widely applied wheat and could make a significant contribution breeding new varieties by saving time increasing selection efficiency doubled (DH) lines. During development system into routine technique, problems been solved (induction medium, genotype dependence, carbohydrate source, applications, etc.). Induction medium important factors for induction subsequent embryoids plantlets. The potato (P-4) published Ouyang et al. (1983), seems effective after our minor modifications (P-4mf, Table 2.9-1). amino acids glutamine asparagine shown enhance they partially replace extract medium. Serine has reputedly positive effect wall Nicotiana culture. Marsolais (1986) others, described an method green plant barley (Hordeum vulgare L.) (Triticum aestivum using Ficoll media. prevents anthers from sinking avoids presence inhibitors agar leading improved androgenic response. Its application opened up chapter Among conditions variables used system, incubation temperature stage microspore are factors. Cold pretreatment breaks down normal (gametophytic pathway) increases frequency symmetrical mitoses, which causes further increase appearance spontaneous formation. Short heat shock treatments at initiation also effective. replacement sucrose other sugars, notably maltose, enhances success Experiments cereals generally embryo or improvement regeneration use maltose instead (Orshinsky al., 1990).