Effect of Pentoxifylline on Tumor Suppressor and Proto-Oncogene Apoptosis in Sperm

摘要: Purpose: Pentoxifylline (PTX), a methylxanthine phosphodiesterase inhibitor reduces superoxide anions responsible for DNA apoptosis. The null hypothesis was that PTX equally effective in reducing damage to specific cell genes. objective determine the integrity of BRCA1 tumor suppressor gene and c-myc proto-oncogene after PTX. Methods: Sperm (64 samples, 4 patients) were preincubated either 0 (control) or 3.6 mM (30 min), washed incubated h at 37 40°C heat shock activation. Single primer polymerase chain reactions (PCR) carried out on lysed sperm targeting exon 11 1. Control single-stranded (ssDNA) stained with 9 μM Hoechst 33342 (blue) while PTX-treated ssDNA SYBR Gold (green). Nytran membrane discs control hybridized PTX-derived ssDNA. Fluorescent images stored microarray design analyzed using ANOVA Students' t-test (P < 0.05) significance. Results:BRCA1 higher pretreatment (93.3 + 10.4 vs. 50.5 9.2; mean SEM). In contrast, there no difference (56.8 9.0 41.7 6.4). Sense antisense primers gave similar fragmentation results. Conclusions: data showed protected but not suggesting did protect different A possible explanation proto-oncogenes had more fragile sites. study involved disc chip assay assess separate PCR-amplified sense strands. results suggested both strands affected by pretreatment.