Involvement of post‐translational modification of neuronal plasticity‐related proteins in hyperalgesia revealed by a proteomic analysis

摘要: To clarify roles of an endogenous pain modulatory system the central nervous (CNS) in hyperalgesia, we tried to identify qualitative and quantitative protein changes by a proteomic analysis using animal model hyperalgesia. Specifically, first induced functional hyperalgesia on male Wistar rats repeated cold stress (specific alternation rhythm temperature, SART). We then compared proteomes multiple regions CNS dorsal root ganglion between hyperalgetic non-treated ones 2-D PAGE pI range 4.0-7.0. found that SART changed prominently mesencephalon cerebellum. thus analyzed two brain more detail gels with narrower ranges. As result, 29 23 spots were significantly cerebellum, respectively. successfully identified 12 MALDI-TOF/TOF MS subsequent database searching. They included unc-18 homolog 67K, collapsin response mediator (CRMP)-2 CRMP-4, which reported be involved neurotransmitter release or axon elongation. Interestingly, mRNA expression levels these three proteins not induction Instead, detected are caused post-translational modification (PTM) proteolysis phosphorylation. Taken together, development would linked PTM proteins. regulation may one useful ways treat