Isolation and sequence of a human cytochrome P-450 cDNA clone.

摘要: Abstract A previously reported cDNA clone [pP450(1)] coding for a phenobarbital-inducible cytochrome P-450 variant of rat liver microsomal membranes, designated P-450e(U.C.), was used as specific hybridization probe to screen human library. Restriction mapping showed that two the colonies isolated contained plasmids overlapping regions same sequence. The [pHP450(1)] having longer insert (1.25 kilobase pairs) sequenced. homology between and cDNAs is 62% in their but only random (24%) 3'-noncoding nucleotides. amino acid sequence deduced from 50% identical P-450e(U.C.). increases 72% if conservative changes residues are permitted. hydropathy profile polypeptide encoded by pHP450(1) almost Regions known be highly conserved isozymes rat, rabbit, mouse were found derived pHP450(1). Analysis Southern blotting indicated part multigene family.