The respiration of protoplasts from leaves of barley infected by powdery mildew (Erysiphe graminis F.SP. Hordei)

摘要: Infection of barley leaves by powdery mildew (Erysiphe graminis f.sp. hordei) causes increased dark respiration, par tof which is associated with active host responses to infection, and a consequence reduced plant growth. The pathogen cannot be grown separately from the host. Therefore, in order examine those changes respiratory activity peculiar host, attempts were made isolate protoplasts infected tissues, healthy controls. Isolation useful numbers (> 106cm−6) viable mesophyll tissues was possible one among several batches commercial ‘Cellulysin’ tested; on analysis sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), this batch contained low molecular weight protein at 20.1 kDa not found other batches. In all isolated protoplasts, total respiration age source-leaf, but within 24 h inoculation stimulated infection. Protoplasts showed small initial increase cyanide (CN)-sensitive component (cytochrome pathway) an CN-insensitive (alternative major cause stimulation respiration.