The role of nitric oxide in the process of implantation in rats

摘要: Nitric oxide (NO) plays an important role in cell signalling many physiological systems, including reproduction. During pregnancy, oestrogen modulates uterine NO generation, and NO may play intermediary the oestrogen-mediated effects on the uterus. Since is actively involved inducing endometrial receptivity to support process of implantation, implantation rats was investigated. N\g=w\-nitro-l-argininemethyl ester (l-NAME), inhibitor NO synthase (NOS), was administered utero with or without sodium nitroprusside (SNP), a generator NO, on different days during preimplantation phase gestation. The status gestation respect failure, embryo development were assessed. l-NAME at various doses (2\p=n-\5mg per uterine horn) pregnancy (days 2\p=n-\6of pregnancy) to optimize the pregnancy terminating dose (absence site day 8 and the effective treatment. led failure when administered at 2.5 mg per horn 3 pregnancy. The characteristic preimplantation permeability changes uterus significantly attenuated embryo growth was retarded. l-NAME-mediated reversed SNP (100 \g=m\g)was co-administered l-NAME. These findings suggest a for the process implantation. possible mechanism by which inhibition NO\p=n-\NOS system interfere discussed.